Rawad Khalaf Hameed, Rafea Zaidan Al-Sugmiany, Hisham Majeed Shlash and Maan Hasan Salih

Department of Biology, College of Science, Tikrit University, Iraq

*Corresponding author: rawad.kh.hameed@tu.edu.iq

Received:  May 10, 2022/ Revised: June 18, 2022 / Accepted: June 21, 2022

Abstract

The study objective was to evaluate the effects of exposure Vicia faba to Wi-Fi waves on growth and molecular levels. The seeds of the Vicia faba plant were soaked for 48 hours, then the seeds were divided into five groups, four for treatments and one for the control group, phenotypic characteristics were recorded, while the molecular markers were evaluated using RAPD-PCR markers to detect the effect of waves on DNA sequence. Morphological study, the highest plant height recorded in treatment B that was 123.9 followed by C, D and E. As for the bean pod number, the treatment E was 13.6 that showed the highest number that followed by D, C and B. For the seed number, the highest was E 34.4 seed that followed by D, B and C treatments. The number of leaves in the plants, the highest was in treatment B that was 251 followed by C, D and E treatments respectively. The leaf measurements recorded, the highest leaf area was in the treatment E that was 3.2-6.1 followed by D, C and B treatments respectively. Most of the treatments were distinguished by unique bands and absent of all mutant bands (51), unique bands (33) and absent bands (18), the treatment E (60) day got a higher number of unique bands that were (12), and the treatment B (15) day have got (4) bands, while the absent bands the treatment D (45) day got a higher number of the absent bands that were (6), and the treatment B (15) day got (4) bands. There was a difference in the results between the morphological and molecular markers because of the difference in mutation frequency on the genome of their coding and noncoding DNA. The Wi-Fi waves induced mutations in the genome of Vicia faba L. and the mutations increased with the period of exposure of the plant to the Wi-Fi waves. The efficiency of the RAPD markers was high in detecting the producing mutation in the genome of Vicia faba L

Keywords  Phenotypic, Molecular Markers, Vicia faba, Wi-Fi Waves

How to cite this article

Hameed, R.K., Al-Sugmiany, R.J., Shlash, H.M.,  and Salih, M.H. (2022). Detecting the effects of Wi-Fi waves on phenotypic and molecular markers of Vicia faba L. Science Archives Vol. 3 (2), 1113–119. http://dx.doi.org/10.47587/SA.2022.3206

References

Ahloowalia, B. S., & Maluszynski, M. (2001). Induced mutations–A new paradigm in plant breeding. Euphytica118(2), 167-173.

Al-doury, S. M., Al-Nasrawi, M. A., & AL-Samarraie, M. Q. (2019). The molecular sequence of Giardia lamblia by using (tpiA) and (tpiB). International Journal of Drug Delivery Technology, 9(03), 374-377.

Al-Ghamdi, S . Hammoud (2009). Application of biotechnology in the improvement of field crops ((municipal beans)). PhD thesis. Faculty of Science of Food and Agriculture-King Saud University.  Saudi Arabia.

Al-Qaisi, Imad Khalaf Khader (2014). The uranium dimension in the strains of maize and its cross hybrids based on the indicators of the RAPD. Tikrit University Journal of Agricultural Sciences. Proceedings of the Third Specialized Conference.

AL-Samarraie, M. Q., AL-Obaedi, A. I., Hamed, N. M., & AL-Azzawie, A. F.(2021). Molecular Identification of Aspergillus nigar Using Randomly Amplified Polymorphic Deoxyribonucleic Acid Polymerase Chain Reaction Technique. Journal of Drug Delivery Technology, 11(4), 1221-1224.

AL-Samarraie, M. Q., Yaseen¹, A. H., & Ibrahim, B. M. (2019). Molecular study of polymorphism for gene tnf-alpha using arms-pcr technique for patients with rheumatoid arthritis. Biochem. Cell. Arch. 19, 4285-4290.

Al-Sugmany, R. Mukhlif (2017). The genetic diversity of a number of beans plant (Faba Vicia) genetic compositions and their individual hybrids are loaded using RAPD PCR technology. Journal of the Tikrit University for Agricultural Sciences-Special issue of the Sixth Scientific Conference of Agricultural Sciences.

 Al-Sugmiany, R. Z., Al-Obaedi, A. I., Al-Jibouri, H. M., & AL-Azzawie, D. F. Using the static magnetic field to generate mutations in the vicia fabagenome and molecular detection with rapd-pcr marker.‏ Plant Archives, 20 (2), 6627-6634.

Al-Sugmiany, R. Z., Saleh, R. F., Mahmood, W. S., & Sadiq, S. T. Study of Wi-Fi waves effects on Genetic variations of Providencia stuartii bacteria isolated from otitis media infections.‏

Al-Zuhiri, N. Ali (2014). The nature of the work of genes using individual, triple and marital camel species between pure breeds of maize (Zea mays L.) and the prediction of marital camel specifications. PhD thesis, Faculty of Agriculture and Forestry, University of Mosul.

Arlius, F., Putri, R. E., Putri, N. S., & Putri, I. (2021, May). Effect of Acoustic Waves on the Growth and Productivity of Sawi Plants (Brassica Juncea L.). In IOP Conference Series: Earth and Environmental Science (Vol. 757, No. 1, p. 012021). IOP Publishing.

Beaubois, E., Girard, S., Lallechere, S., Davies, E., Paladian, F., Bonnet, P., … & Vian, A. (2007). Intercellular communication in plants: evidence for two rapidly transmitted systemic signals generated in response to electromagnetic field stimulation in tomato. Plant, cell & environment30(7), 834-844.‏

Blair, M. W., Díaz, L. M., Buendía, H. F., & Duque, M. C. (2009). Genetic diversity, seed size associations and population structure of a core collection of common beans (Phaseolus vulgaris L.). Theoretical and Applied Genetics119(6), 955-972.

Gorbāns, I., & Jurenoks, A. (2019). Some Aspects of Good Practice for Safe Use of Wi-Fi, Based on Experiments and Standards. Applied Computer Systems24(2), 161-165.

Huang, Q. X., Wang, X. C., Kong, H., Guo, Y. L., & Guo, A. P. (2013). An efficient DNA isolation method for tropical plants. African Journal of Biotechnology12(19).

Hussein, J. K. (2016). Induction of mutagenesis by Gamma radiation and their early evaluation by RAPD on Lathyrus odoratus L. Kufa Journal for Agricultural Sciences8(3).

 Laskar, R. A., Khan, S., Khursheed, S., Raina, A., & Amin, R. (2015). Quantitative analysis of induced phenotypic diversity in chickpea using physical and chemical mutagenesis. Journal of Agronomy14(3), 102.

 Liptai, P., Dolník, B., & Gumanová, V. (2017). Effect of Wi-Fi radiation on seed germination and plant growth-experiment. Annals of the Faculty of Engineering Hunedoara15(1), 109.

Multari, S., Stewart, D., & Russell, W. R. (2015). Potential of fava bean as future protein supply to partially replace meat intake in the human diet. Comprehensive Reviews in Food Science and Food Safety14(5), 511-522.‏

Mustafa, M. A., AL-Samarraie, M. Q., & Ahmed, M. T. (2020). Molecular techniques of viral diagnosis. Science Archives, 1(3), 89-92.

Rivero, R. M., Mittler, R., Blumwald, E., & Zandalinas, S. I. (2022). Developing climate‐resilient crops: improving plant tolerance to stress combination. The Plant Journal109(2), 373-389.‏

Roux, D., Vian, A., Girard, S., Bonnet, P., Paladian, F., Davies, E., & Ledoigt, G. (2006). Electromagnetic fields (900MHz) evoke consistent molecular responses in tomato plants. Physiologia plantarum128(2), 283-288.‏

Saleh RF, Al-Sugmiany RZ, Al-Doori MM, Al-Azzawie A. (2020). Phenotypic and Genetic Effects of Wi-Fi Waves on Some Bacterial Species Isolated from Otitis Media Infection Tropical Journal of Natural Product Research, 4(12),1056-1063.

Sambrook, J., Fritch, E. F. and Maniatis, J. (1989) Molecular cloning, a laboratory Manual. 2nd edition. Cold spring Harbor laboratory press, New York.

Singh, H. P., Sharma, V. P., Batish, D. R., & Kohli, R. K. (2012). Cell phone electromagnetic field radiations affect rhizogenesis through impairment of biochemical processes. Environmental Monitoring and assessment184(4), 1813-1821.‏

Sivani, S., & Sudarsanam, D. (2012). Impacts of radio-frequency electromagnetic field (RF-EMF) from cell phone towers and wireless devices on biosystem and ecosystem-a review. Biology and Medicine4(4), 202.‏

Tkalec, M., Malarić, K., & Pevalek-Kozlina, B. (2007). Exposure to radiofrequency radiation induces oxidative stress in duckweed Lemna minor L. Science of the Total Environment388(1-3), 78-89.‏

Treviño-Castellano, M., Rodríguez-Nóvoa, S., Llovo-Taboada, J., García-Zabarte, A., García-Riestra, C., & Regueiro-García, B. J. (2003). Combined used of RAPD and touchdown PCR for epidemiological studies of Aspergillus fumigatus. Enfermedades infecciosas y microbiologia clinica21(9), 472-476.

Vian, A., Davies, E., Gendraud, M., & Bonnet, P. (2016). Plant responses to high frequency electromagnetic fields. BioMed research international2016.‏

Weigand, F., Baum, M., & Udupa, S. (1993). DNA molecular marker techniques. Technical Manual No. 20. ICARDA.

Williams, J. G., Kubelik, A. R., Livak, K. J., Rafalski, J. A., & Tingey, S. V. (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic acids research18(22), 6531-6535.

Zamanian, A., & Hardiman, C. J. H. F. E. (2005). Electromagnetic radiation and human health: A review of sources and effects. High Frequency Electronics4(3), 16-26.‏

Zhang, X., Lei, L., Lai, J., Zhao, H., & Song, W. (2018). Effects of drought stress and water recovery on physiological responses and gene expression in maize seedlings. BMC plant biology18(1), 1-16.

Licence                  Article Metadata

This work is licensed under a Creative Commons Attribution 4.0 International License.

[pdf_attachment file="1" name="View Details"]