Sarita Mishra, Rakhi Agarwal
Laboratory of Analytical & Molecular Toxicology (Forensic Chemistry & Toxicology Laboratory)
School of Forensic Science, National Forensic Sciences University, Gujarat, India
Received: Jan 25, 2021 / Revised: Feb 25, 2021/ Accepted: Mar 22, 2021
Abstract
Exposure to various xenobiotics and microbial infections raise serious health concerns including the several phenotypic and behavioral anomalies in the exposed animals. Thus, an early assessment of altered behavioral endpoints will help to identify the toxic nature of a xenobiotic compound and its adverse impacts on the living organism. Several model organisms are being used for the toxicity assessment of various xenobiotics. C. elegans, a worm, possess several behaviors such as pharyngeal pumping, locomotion (speed, body bending), etc. Thus, by examining the alterations in such parameters, one can easily assess the behavioral abnormalities associated with toxicants/infections. In the present study, we have demonstrated a stepwise, simplified version of pharyngeal pumping and the locomotion assays (speed, body bending, omega turn, and reversal locomotion behavior) that can be easily performed in the laboratory for the early health assessment.
Keywords Rapid Assessment, Early Biomarker, Toxicant exposure, Alternate Model, C. elegans, Locomotion, and Pharyngeal pumping behavior
How to cite this article:
Mishra S., Agarwal R. (2021). An Optimized Assay for Early and Rapid Assessment of Behavioral Alterations using Caenorhabditis elegans as an alternate Animal Model Science Archives, Vol. 2 (1), 56-61 http://dx.doi.org/10.47587/SA.2021.2109
Copyright
This is an open-access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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