Omar Raheem Khalaf AL-Obaidi1, Mohammed Ahmed Mustafa2,3, Rasha Abdul Adheem Yaseen1
1College of Education, Biology Department, University of Samarra, Iraq
2Department of Pathological Analysis, College of Applied Sciences, University of Samarra, Iraq
3Department of Medical Laboratory Techniques, College of Technology, University of Imam Jafar Al-Sadiq Dujail
*Corresponding author: mohammed.alsad3@gmail.com
Received: Feb 10, 2021 / Revised: Mar 9, 2021/ Accepted: Mar 15, 2020
Abstract
Next-generation sequencing (NGS) is a sort of DNA sequencing innovation that utilizes equal sequencing of numerous little sections of DNA to decide succession. As opposed to Sanger sequencing, the speed of sequencing and measures of DNA grouping information created with NGS, which is viewed as a “high-throughput innovation”, are dramatically more prominent, and are delivered at sLJnLficDntl\ diminished expenses. NGS opens the whole range of genomic adjustments for the hereditary examination of complex characteristics. The primary test in the quickly developing HT-NGS advances is to adapt to the investigation of the huge creation of sequencing data set through cutting-edge bioinformatics apparatuses.
Keywords Next-generation sequencing, PCR, DNA sequencing
How to cite this article
AL-Obaidi, ORK., Mustafa, M.A., Yaseen, R.A.A. (2021). Next-generation sequencing techniques. Science Archives, Vol. 2 (1), 26-29.
http://dx.doi.org/10.47587/SA.2021.2105
References
Adams, J. (2008). DNA sequencing technologies. Nature Education, 1, 193.
Benner, S., Chen, R.J., Wilson, N.A., Abu-Shumays, R., Hurt, N., Lieberman, K.R. et al. (2007). Akeson M. Sequencespecific detection of individual DNA polymerase complexes in real time using a nanopore. Nat Nanotechnol, 2(11),718–724.
Bokulich, N. A., Bergsveinson, J., Ziola, B., and Mills, D. A. (2015). Mapping microbial ecosystems and spoilage-gene flow in breweries highlights patterns of contamination and resistance. Elife, e04634.
Branton, D., Deamer, D.W., Marziali, A., Bayley, H., Benner, S.A., Butler, T. et al. (2008). Schloss JA. The potential and challenges of nanopore sequencing. Nat Biotechnol. 26(10),1146–1153.
Chakravorty, S., Helb, D., Burday, M., Connell, N., and Alland, D.A. (2007). Detailed analysis of 16S ribosomal RNA gene segments for the diagnosis of pathogenic bacteria. J. Microbiol. Methods, 69, 330–339.
Chandra, S.P., Rafal, S., Andrzej, T. (2011). Sequencing technologies and genome sequencing. J Appl Genet, 52, 413-435.
Cherf, G.M., Lieberman, K.R., Rashid, H., Lam, C.E., Karplus, K., Akeson, M. (2012). Automated forward and reverse ratcheting of DNA in a nanopore at 5-A precision. Nat Biotechnol, 30(4),344–348.
Claesson, M. J., O’Sullivan, O., Wang, Q., Nikkil,ä, J., Marchesi, J. R., Smidt, H., et al. (2009). Comparative analysis of pyrosequencing and a phylogenetic microarray for exploring microbial community structures in the human distal intestine. PLoS ONE, 4:e6669.
Edgar, R. C. (2010). Search and clustering orders of magnitude faster than BLAST. Bioinformatics, 26, 2460–2461.
Giovannoni, S. J., Britschgi, T. B., Moyer, C. L., and Field, K. G. (1990). Genetic diversity in Sargasso sea bacterioplankton. Nature, 345, 60–63.
Hanna, G.J., Johnson, V.A., Kuritzkes, D.R., Richman, D.D., Martinez-Picado, J., Sutton, L., et al. (2000). Comparison of sequencing by hybridization and cycle sequencing for genotyping of human immunodeficiency virus type 1 reverse transcriptase. J Clin Microbiol, 38(7), 2715–2721.
Kable, M. E., Srisengfa, Y., Laird, M., Zaragoza, J., Mcleod, J., Heidenreich, J., et al. (2016). The core and seasonal microbiota of raw bovine milk in tanker trucks and the impact of transfer to a milk processing facility. MBio, 7, 1–13.
Liu, Z., Desantis, T. Z., Andersen, G. L., and Knight, R. (2008). Accurate taxonomy assignments from 16S rRNA sequences produced by highly parallel pyrosequencers. Nucleic Acids Res, 36, 1–11.
Liu, Z., Wang, Y., Deng, T., Chen, Q. (2016). Solid-State Nanopore-Based DNA Sequencing Technology. Journal of Nanomaterials, 13.
Mardis, E.R. (2008). The impact of next-generation sequencing technology on genetics. Trends Genet, 3, 133-141.
Mustafa, M.A., AL-Samarraie M.Q., Ahmed M. T. (2020). Molecular techniques of viral diagnosis, Science Archives, 1(3), 89-92 http://dx.doi.org/10.47587/SA.2020.1303.
Mustafa, M.A & AL-Samarraie, M.Q. (2020) .Secondary menopause and its relationship to hormonal levels among women at salah al-din hospital. European Journal of Molecular & Clinical Medicine. 7 (9), 96-104.
Neefs, J. M., Van de Peer, Y., De Rijk, P., Chapelle, S., and De Wachter, R. (1993). Compilation of small ribosomal subunit RNA structures. Nucleic Acids Res, 21, 3025–3049.
Qin, Y., Schneider, T.M., Brenner, M.P. (2012). Sequencing by hybridization of long targets. PLoS One, 7(5), e35819.
Rizzo, J.M. and Buck, M.J. (2012). Key principles and clinical applications of “next-generation” DNA sequencing. Cancer Prev Res (Phila), 5, 887-900.
Rodrigues, M. X., Lima, S. F., Canniatti-Brazaca, S. G., and Bicalho, R. C. (2017).The microbiome of bulk tank milk: characterization and associations with somatic cell count and bacterial count. J. Dairy Sci, 100, 2536–2552.
Schadt, E.E., Turner, S., Kasarskis, A. (2010). A window into third-generation sequencing. Hum Mol Genet, 19: R227-R240.
Schloss, P.D., Westcott, S. L., Ryabin, T., Hall, J. R., Hartmann, M., Hollister, E. B., et al. (2009).Introducing mothur: open-source, platformindependent, community-supported software for describing and comparing microbial communities. Appl. Environ. Microbiol, 75, 7537–7541.
Venter, J.C., Adams, M.D., Myers, E.W., Li, P.W., Mural, R.J. et al. (2001). The sequence of the human genome. Science, 291, 1304-1351.
Voelkerding, K.V., Dames, S.A., Durtschi, J.D. (2009). Next-generation sequencing: from basic research to diagnostics. Clin Chem, 55, 641-658.
Wang, Q., Garrity, G. M., Tiedje, J. M., and Cole, J. R. (2007). Naïve Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl. Environ. Microbiol, 73, 5261–5267.
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