Debarati Roy, Mousumi Dutta, Mukti Mondal, Kaushik Sarkar, Goutam Paul*
Molecular Neurotoxicology Laboratory, Department of Physiology,
University of Kalyani, Kalyani, Nadia-741235, West Bengal, India
*Corresponding author: goutampaul.ku@gmail.com
Received: May 31, 2021 / Revised: June 23, 2021/ Accepted: June 27, 2021
Abstract
Bisphenol S (BPS) being a component of storage and packaging materials of foodstuffs, beverages and drinking water, contaminates them through BPS-leaching. BPS exposure to humans occurs predominantly through the consumption of BPS-intoxicated foodstuffs and subsequently BPS absorption through the small intestine into bloodstream. Though, the probable toxic effects of BPS on contractile function of small intestinal visceral smooth muscle (VSM) that provides motility to the small intestine, remains poorly understood. So, the present study aims to evaluate the effects of BPS on the efferent neurocrine mechanisms involved in the intrinsic rhythmic contraction of small intestinal VSM. Experiments were performed on duodenum segment of small intestine isolated from adult male Sprague-Dawley rats to examine the effects of BPS exposure on the movement of the duodenum, acetylcholinesterase (AChE) activity, levels of cellular oxidative stress biomarkers, and activities of cytosolic antioxidant enzymes at 100µM, 200µM, 400µM and 800µM doses of BPS for 1h duration ex vivo by using standard laboratory protocol. We observed significant decrease in frequency and amplitude of contraction of duodenum recorded ex vivo in single-dose acute exposure experiments in BPS-exposed groups compared to the control group (P<0.05). Besides, acetylcholinesterase (AChE) activity in duodenal homogenate has been increased significantly along with significant alterations in both the levels of oxidative stress biomarkers and activities of antioxidant enzymes in a dose-response manner in BPS-exposed groups (P<0.001). Our results indicate that BPS inhibits contraction of duodenal VSM probably by inhibiting the activity of excitatory cholinergic myenteric efferents through inducing enzymatic activity of AChE and promoting oxidative stress in duodenum.
Keywords Bisphenol S, Duodenal visceral smooth muscle, Cholinergic myenteric efferent, AChE activity, Oxidative stress
How to cite this article:
Roy, D., Dutta, M., Mondal, M., Sarkar, K., Paul, G. (2021). Effect of Bisphenol S (BPS) on the contraction of duodenal visceral smooth muscle ex vivo in rat. Science Archives, Vol.2(2),99-108. http://dx.doi.org/10.47587/SA.2021.2207
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