Akeel Shamran Tushy
, Abdul-A Deem Y. Al.- Barrak, Nawras Noori Al-Alawae
1- Center for Gastroenterology and Hepatology, Clinical Immunology Unit, Ministry of Health, Department of Health, Babylon, Iraq
2-Faculty of Medicine, Al-Mustansiriya University, Iraq
Article History: Received: Nov 08, 2020 / Revised: Dec 03, 2020/ Accepted: Dec 16, 2020
Abstract
This study was aimed at assessing NK cell cytotoxicity, soluble Fas ligand (sFasL) serum concentration among Toxoplasmosis patients, and healthy controls. To understanding the role of these parameters in the immunity and pathophysiology of toxoplasmosis, correlations between these parameters were evaluated. NK cell cytotoxicity was estimated by the cultivation of Candida cells on Sabouraud`s dextrose agar (SDA) plate which had been incubated for two hours with NK cells. Colony-forming inhibition assay (CFIA) by calculating anticandidal index (ACI) has been estimated in two ratios of target: effector (T: E) cells 1:3 (NK R1) and 1:30 (NK R2) for both patients and controls. Enzyme-linked immunosorbent assay (ELISA) kit sFasL were used to estimate serum level in both patients and controls. There is a decrease in sFasL in toxoplasmosis compared to controls.
Keywords Toxoplasmosis, Apoptosis, FAS/FASL, NK Cells, Cytotoxicity
How to cite this article
Tushy, A.S., Abdul., A Deem, Y. Al.- Barrak, & Al-Alawae, N.N. (2020). Impact of toxoplasmosis on apoptotic activity and natural killer cell cytotoxicity among Iraqi pregnant women. Science Archives, Vol. 1 (3), 123-131.
Crossref DOI
http://dx.doi.org/10.47587/SA.2020.1309
Copyright
This is an open-access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium provided the original work is properly cited.
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