Amita Gaurav Dimri
, Abhishek Chauhan
M.L Aggarwal
1-Deaprtment of microbiology, Shriram Institute for Industrial Research, 19, University Road, Delhi-11007
2-Amity Institute of Environmental Toxicology, Safety and Management, Amity University, Sector-125, Noida, Uttar Pradesh, India
Received Date: Jan 24, 2020 / Accepted Date: June 22, 2020 / Published Date: June 27, 2020
Abstract:
In the present investigation, Streptosporangium sp. (AI-21) a new soil borne isolate was characterized against food borne bacteria isolated from thirty-five food samples. Based on the morphological and biochemical characteristics, it was found that all food borne bacteria belong to Escherichia, Pseudomonas, Salmonella, Staphylococcus and Bacillus group of microorganisms. Colony morphology and biochemical characterization was the base of identification of actinomycete species. The actinomycete isolate was grown at a mass level by inoculum build up in mother flasks. Extraction of antibacterial substance from isolated actinomycetes was done by solvent- extraction method. Ethyl acetate extract exhibited activity against Staphylococcus sp. and Escherichia sp. The MICs of this extract was recorded in the range of 512 μg/ml to 2048 μg/mL. A maximum zone of 23.15mm was observed against Staphylococcus sp. The study not only targeted food borne isolates but also suggested how soil could be explored for the extraction of bioactive molecules by actinomycetes. This study could be a platform for new drug discovery.
Keywords Food borne bacteria, soil actinomycetes, antimicrobial compounds and drug discovery
How to cite this article
Dimri, A.G., Chauhan, A., Aggarwal, M.L. (2020) In vitro antibacterial characterization of Streptosporangium sp. (AI-21) a new soil isolate against food borne bacteria. Science Archives, Vol. 1 (1), 25-34.
Crossref DOI
http://dx.doi.org/10.47587/SA.2020.1103
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This is an open-access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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