Amira Ismail Al Badrani and Khansa Mohammed Younis ✉
Department of Biology/Microbiology, Faculty of Sciences, Mosul University, Mosul, Iraq
Received: Sept 2, 2022/ Revised: Oct 2, 2022/ Accepted: Oct 5, 2022
(✉) Corresponding Author: kansbio50@uomosul.edu.iq
Abstract
One of the characteristics that distinguish living things is communication or the simplest form of talking and sharing information. Gram-negative bacteria use the AHL autoinducer for their quorum sensing, and this quorum sensing has been a major factor in the development of pathogenic conditions in a specific host. This study aimed to isolate, identify A. baumannii, and detect N-acyl-homoserine lactone (AHL) produced by A. baumannii, which was isolated from patients admitted to Mosul hospitals in Iraq. A total of 125 samples were taken from patients who were being treated at Mosul hospitals. The isolates were characterized using the Vitek-2 and API 20 E systems as well as microscopy and biochemistry. Only 25 isolates were confirmed as A. baumannii. Patients with burns had the highest prevalence of A. baumannii (40%) while those with blood and throat swabs had the lowest prevalence (8%). This study assessed the sensitivity of all A. baumannii isolates to thirteen different antibiotic classes using the disc diffusion method. The three categories of A. baumannii infection’s antibiotic sensitivity (MDR), extensively drug-resistant (XDR), and non-multi-drug resistance (non-MDR) were calculated to be, respectively, 34.61%, 53.84%, and 11.53. The -galactosidase assay was used to quantify AHL activity during a 24-hour growth phase in order to determine the association between AHL production by A. baumannii isolates and their phase’s growth. Using the biosensor strain Agrobacterium tumefaciens KYC55, all isolates displayed the quantitative and qualitative activity of acyl homoserine lactones (AHL) signals.
Keywords: Acinetobacter baumannii, MDR, Quorum Sensing, Agrobacterium tumefaciens
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How to cite this article
Al-Badrani, A. I., Younis, K. M. (2022). Isolation and Evaluation of Acinetobacter baumannii from hospitals in Mosul city and detect their ability to produce acylated homoserine lactones. Science Archives, Vol. 3(4), 247-254. https://doi.org/10.47587/SA.2022.3402
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