Mohammed Ahmed Mustafa
, Marwan Q AL-Samarraie & Marwa T. Ahmed
Department of Pathological Analysis, College of Applied Sciences, University of Samarra
Department of Medical Laboratory Techniques, College of Technology, University of Imam Jafar Al-Sadiq Dujail
Department of Microbiology, College of Medicine, University of Tikrit, Iraq
mohammed.alsad3@gmail.com
Article History: Received: Nov 19, 2020 / Revised: Dec 2, 2020/ Accepted: Dec 11, 2020
Abstract
Viral infections are the cause of very serious problems in humans all over the globe. The recent outbreak of coronavirus disease 2019 caused by SARS-CoV-2 is a perfect example of how viral infection could pose a great threat to global public health and economics. Therefore, to defeat the viral pathogens, we need to get a reliable diagnosis. Detection of the viral presence fast and accurate in the patient is important for appropriate treatment, control, and prevention of epidemics. Diagnosis of infectious microbes became easier by the development of molecular techniques, especially with the emerging of the polymerase chain reaction (PCR). The easiness and high sensitivity of the technique made this method reliable enough to be used to detect any known genetic sequence has led to its wide application in the life sciences. Currently, real-time PCR has made remarkable contributions, with an additional fluorescent probe detection system that has increased the sensitivity of this assay over conventional PCR, with the ability to verify the amount of amplification product and to quantitate the target concentration. Further, nucleotide sequence analysis of the amplification products has encouraged epidemiological studies of contagious disease in outbreaks, and the follow up of treatment for infections, particularly the viruses with a high tendency to mutations.
Keywords Virus, Molecular diagnosis, Molecular microbiology, Nucleic acid, PCR techniques, Viral laboratory diagnosis
How to cite this article
Mustafa, M.A., AL-Samarraie M.Q., Ahmed M. T. (2020). Molecular techniques of viral diagnosis, Science Archives, 1 (3), 98-101.
Crossref DOI
http://dx.doi.org/10.47587/SA.2020.1304
Copyright
This is an open-access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium provided the original work is properly cited.
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