Phytochemical screening and preliminary evaluation of antioxidant activity of three Indonesian Araucaria leaves extracts

Kurniawanti¹, Dhea Demitri Agusta¹, Hanhan Dianhar², Dyah Utami Cahyaning Rahayu³ Purwantiningsih Sugita^1*                                                                 

 ¹Department of Chemistry, Faculty of Mathematics and Natural Sciences (FMIPA), IPB University,

Bogor 16680, West Java, Indonesia

²Chemistry Study Program, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Jakarta,

Jakarta 13220, DKI Jakarta, Indonesia

³Department of Chemistry, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Indonesia,

Depok 16424, West Java, Indonesia

*Corresponding author:  purwantiningsih@apps.ipb.ac.id

Received: Aug 22, 2021 / Revised: Sept 24, 2021/ Accepted: Sept 28, 2021

Abstract

 Araucaria is the largest genus in Araucariaceae and is well known as an evergreen coniferous tree. Several species of Araucaria have been used as traditional medicinal plants. This study aims to determine the phytochemical constituents, and preliminary antioxidant activities of three Indonesian Araucaria, i.e. A. hunsteinii, A. columnaris, and A. cunninghamii leaves extracts. All the leaves of Indonesian Araucaria were macerated in acetone to give acetone extracts. A qualitative phytochemical screening method was used to determine the class of secondary metabolites in the three leaves extracts, including alkaloids, flavonoids, steroids, terpenoids, saponins, and tannins. In addition, the preliminary antioxidant activity of the corresponding extract was determined by spraying DPPH (2,2-diphenyl-1-picrylhydrazyl) to the TLC plate, which will turn a yellow spot as a positive result. The yield of acetone extract of A. columnaris, and A. cunninghamii, A. hunsteinii were 0.85%, 1.64%, and 3.07%, respectively. Phytochemical analysis showed that all leaves acetone extracts contained flavonoids, steroids, terpenoids, phenolics, and tannins. Furthermore, the strongest to the weakest antioxidant activities were summarized as follows A. hunsteinii, A. columnaris, and A. cunninghamii, sequentially. These preliminary results reveal that all Indonesian Araucaria leaves extracts are potential for further study.

Keywords  Araucaria, Araucariaceae, phytochemical, antioxidant, DPPH

How to cite this article:

Kurniawanti, Agusta, D. D., Dianhar, H., Rahayu, D. U. C., Sugita, P. (2021). Phytochemical Screening and Preliminary Evaluation of Antioxidant Activity of Three Indonesian Araucaria Leaves Extracts. Science Archives, Vol. 2(3), 250-254 . http://dx.doi.org/10.47587/SA.2021.2316

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