Raina Ghosh, Sourapriya Mukherjee, Kaushik Sarkar, Goutam Paul*
Molecular Neurotoxicology Laboratory, Department of Physiology, University of Kalyani,
Kalyani – 741235, West Bengal, India
*Corresponding author: goutampaul.ku@gmail.com
Received: Aug 06, 2021 / Revised: Sept 09, 2021/ Accepted: Sept 15, 2021
Abstract
Methylparaben, a synthetic chemical, is used as preservative to enhance the lifespan of different food items to be stored. So, humans are often exposed to methylparaben through foods preserved with methylparaben. The aim of the study was to examine the effect of methylparaben on contractile function of duodenum, the initial part of small intestine, which helps in digestion and absorption of ingested food. In our study, we have found significant increase in amplitude and frequency of the contraction of duodenum ex vivo in rats exposed to methylparaben dose dependently for all exposure durations in comparison with contraction of the duodenum of rats exposed to DMSO (vehicle control). Significant inhibition of the enzymatic activity of acetylcholinesterase (AChE) in duodenal smooth muscle of exposed rats has also been observed dose dependently in all exposure durations. These results suggest that methylparaben promotes the contraction of duodenum probably by potentiating the contraction of duodenal visceral smooth muscle (VSM). The methylparaben induced potentiation of duodenal VSM might be due to inhibition of AChE activity. The activities of antioxidant enzymes (GPx and GR) have significantly decreased and LDH activity and malondialdehyde (MDA) level have significantly increased in smooth muscle homogenates of rats exposed to methylparaben in our study. These observations suggest that methylparaben inhibits the AChE activity probably by producing oxidative stress in smooth muscle cells. In conclusion, methylparaben potentiates the contractile function of duodenal VSM probably by promoting oxidative stress induced inhibition of AChE activity at myoneural junctions.
Keywords Methylparaben, Duodenal visceral smooth muscle, Oxidative stress, Acetylcholinesterase, Myoneural junction
How to cite this article:
Ghosh, R., Mukherjee, S., Sarkar, K., Paul, G. (2021). Potentiation of the contraction of duodenal visceral smooth muscle in rat through oxidative stress induced inhibition of AChE activity by methylparaben. Science Archives, Vol. 2 (3), 194-200. http://dx.doi.org/10.47587/SA.2021.2307
References
Ahmad A, Rasheed N, Banu N, Palit G. 2010. Alterations in monoamine levels and oxidative systems in frontal cortex, striatum, and hippocampus of the rat brain during chronic unpredictable stress. Stress 13:355–364. https://doi.org/10.3109/10253891003667862
Ahn, H. J., An, B. S., Jung, E. M., Yang, H., Choi, K. C., & Jeung, E. B. (2012). Parabens inhibit the early phase of folliculogenesis and steroidogenesis in the ovaries of neonatal rats. Molecular reproduction and development, 79(9), 626-636. https://doi.org/10.1002/mrd.22070
Baker, B. H., Wu, H., Laue, H. E., Boivin, A., Gillet, V., Langlois, M. F., &Takser, L. (2020). Methylparaben in meconium and risk of maternal thyroid dysfunction, adverse birth outcomes, and Attention-Deficit Hyperactivity Disorder (ADHD). Environment international, 139, 105716. https://doi.org/10.1016/j.envint.2020.105716
Banan, A., Choudhary, S., Zhang, Y., Fields, J. Z., & Keshavarzian, A. (1999). Ethanol-induced barrier dysfunction and its prevention by growth factors in human intestinal monolayers: evidence for oxidative and cytoskeletal mechanisms. Journal of Pharmacology and Experimental Therapeutics, 291(3), 1075-1085.
Belozerskaia TA, Gessler NN. 2007. Reactive oxygen species and the strategy of the antioxidant defense in fungi: A review. PriklBiokhimMikrobiol 43:565–575. https://doi.org/10.1134/S0003683807050031
Bionetics, L. (1974). Mutagenic evaluation of compound FDA 71–38, methyl paraben. US NTIS Report. PB245, 459.
Carmona, E., Andreu, V., &Picó, Y. (2014). Occurrence of acidic pharmaceuticals and personal care products in Turia River Basin: from waste to drinking water. Science of the total environment, 484, 53-63. https://doi.org/10.1016/j.scitotenv.2014.02.085
Colgan, S. P., & Taylor, C. T. (2010). Hypoxia: an alarm signal during intestinal inflammation. Nature reviews Gastroenterology & hepatology, 7(5), 281-287. https://doi.org/10.1038/nrgastro.2010.39
Costa, Janaína R., Mônica S. Campos, Rodrigo F. Lima, Liana S. Gomes, Mara R. Marques, Sebastião R. Taboga, Manoel F. Biancardi, Pedro VA Brito, and Fernanda CA Santos. “Endocrine‐disrupting effects of methylparaben on the adult gerbil prostate.” Environmental toxicology 32, no. 6 (2017): 1801-1812. https://doi.org/10.1002/tox.22403
Daughton, C. G. (2016). Pharmaceuticals and the Environment (PiE): Evolution and impact of the published literature revealed by bibliometric analysis. Science of the Total Environment, 562, 391-426. https://doi.org/10.1016/j.scitotenv.2016.03.109
Devasagayam, T. P., & Tarachand, U. (1987). Decreased lipid peroxidation in the rat kidney during gestation. Biochemical and biophysical research communications, 145(1), 134-138. https://doi.org/10.1016/0006-291X(87)91297-6
Haman, C., Dauchy, X., Rosin, C., & Munoz, J. F. (2015). Occurrence, fate and behavior of parabens in aquatic environments: a review. Water Research, 68, 1-11. https://doi.org/10.1016/j.watres.2014.09.030
Heinemann, A., Pieber, D., & Holzer, P. (2002). Inhibition by female sex steroids of peristalsis in the guinea pig small intestine. Digestion, 65(4),213-219. https://doi.org/10.1159/000063820
Iborra, M., Moret, I., Rausell, F., Bastida, G., Aguas, M., Cerrillo, E., & Beltran, B. (2011). Role of oxidative stress and antioxidant enzymes in Crohn’s disease. Biochemical Society Transactions, 39(4), 1102-1106. https://doi.org/10.1042/BST0391102.
Javurek, A. B., Suresh, D., Spollen, W. G., Hart, M. L., Hansen, S. A., Ellersieck, M. R., & Rosenfeld, C. S. (2017). Gut dysbiosis and neurobehavioral alterations in rats exposed to silver nanoparticles. Scientificreports, 7(1),1-15. https://doi.org/10.1038/s41598-017-02880-0
Joint FAO/WHO Expert Committee on Food Additives. Meeting, & World Health Organization. (2010). Evaluation of Certain Food Additives: Seventy-first Report of the Joint FAO/WHO Expert Committee on Food Additives (Vol. 71). World Health Organization.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of biological chemistry, 193, 265-275.
Matthews, C., Davidson, J., Bauer, E., Morrison, J. L., & Richardson, A. P. (1956). p-Hydroxybenzoic Acid Esters as Preservatives II.: Acute and Chronic Toxicity in Dogs, Rats, and Mice. Journal of the American Pharmaceutical Association (Scientific ed.), 45(4), 260-267. https://doi.org/10.1002/jps.3030450420
Naziroglu M, Akkus S, Soyupek F, Yalman K, Celik O, Eris S, Uslusoy GA. 2010. Vitamins C and E treatment combined with exercise modulates oxidative stress markers in blood of patients with fibromyalgia: A controlled clinical pilot study. Stress 13: 498 –505. https://doi.org/10.3109/10253890.2010.486064
Pérez, R. A., Albero, B., Miguel, E., & Sánchez-Brunete, C. (2012). Determination of parabens and endocrine-disrupting alkylphenols in soil by gas chromatography–mass spectrometry following matrix solid-phase dispersion or in-column microwave-assisted extraction: a comparative study. Analytical and bioanalytical chemistry, 402(7), 2347-2357. https://doi.org/10.1007/s00216-011-5248-0
Rachmilewitz, D., Stamler, J. S., Karmeli, F., Mullins, M. E., Singel, D. J., Loscalzo, J., & Podolsky, D. K. (1993). Peroxynitrite-induced rat colitis—a new model of colonic inflammation. Gastroenterology, 105(6),1681-1688. https://doi.org/10.1016/0016-5085(93)91063-N
Rotruck, J. T., Pope, A. L., Ganther, H. E., Swanson, A. B., Hafeman, D. G., & Hoekstra, W. (1973). Selenium: biochemical role as a component of glutathione peroxidase. Science, 179(4073), 588-590. DOI: 10.1126/science.179.4073.588
Samarasinghe, S. V. A. C., Krishnan, K., Naidu, R., Megharaj, M., Miller, K., Fraser, B., & Aitken, R. J. (2018). Parabens generate reactive oxygen species in human spermatozoa. Andrology, 6(4), 532-541. https://doi.org/10.1111/andr.12499
Sarkar, K., Tarafder ,P., Nath, P.P., Paul, G.(2013). Bisphenol A inhibits duodenal movement in rat by increasing acetylcholinesterase activity and decreasing availability of free Ca2+ in smooth muscle cells. International Journal of Pharma and Bio Sciences, 4(2), 679-688.
Shahare, B., Yashpal, M., & Gajendra. (2013). Toxic effects of repeated oral exposure of silver nanoparticles on small intestine mucosa of mice. Toxicology mechanisms and methods, 23(3), 161-167. https://doi.org/10.3109/15376516.2013.764950
Shen L, Su L, Turner JR. 2009. Mechanisms and functional implications of intestinal barrier defects. Dig Dis 27:443–449. https://doi.org/10.1159/000233282
Smolinske, S. C. (1992). Parabens. In Handbook of food, drug, and cosmetic excipients. CRC Press Boca Raton, FL, pp. 251-258. https://doi.org/10.1201/9781315140513
Staal, G. E., Visser, J., &Veeger, C. (1969). Purification and properties of glutathione reductase of human erythrocytes. Biochimica et Biophysica Acta (BBA)-Enzymology, 185(1), 39-48.
Van Den Brûle, S., Ambroise, J., Lecloux, H., Levard, C., Soulas, R., De Temmerman, P. J., &Lison, D. (2015). Dietary silver nanoparticles can disturb the gut microbiota in mice. Particle and fibre toxicology, 13(1), 1-16. https://doi.org/10.1186/s12989-016-0149-1
Vom Saal, F. S., Cooke, P. S., Buchanan, D. L., Palanza, P., Thayer, K. A., Nagel, S. C., &Welshons, W. V. (1998). A physiologically based approach to the study of bisphenol A and other estrogenic chemicals on the size of reproductive organs, daily sperm production, and behavior. Toxicology and industrial health, 14(1-2), 239-260. https://doi.org/10.1177/074823379801400115
Wróblewski, F., & Ladue, J. S. (1955). Lactic dehydrogenase activity in blood. Proceedings of the society for experimental biology and medicine, 90(1), 210-213. https://doi.org/10.3181/00379727-90-21985
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