Priyanka J. Patel ¹✉ Goral Trivedi ², Shreyas Bhatt¹ and Meenu Saraf ³
¹Department of Life Sciences, Hemchandracharya North Gujarat University, Patan – 384265, India
²Valent BioSciences, Libertyville, IL – 60048, United States
³Department of Microbiology and Biotechnology, University School of Sciences, Gujarat University,
Ahmedabad – 380 009, India.
Received: Dec 28, 2022/ Accepted: Jan 19, 2023/ Published: Jan 30, 2023
(✉) Corresponding Author: pu2989@gmail.com
Abstract
Biofortification is receiving more devotion to enhance the Phyto-availability of micronutrients such as Fe, Zn, and Se, in the major food crops. Isolation of selenorhizobacteria was done on a nutrient agar plate supplemented with Na2SeO3. Among them, the best strain MPJ12 was chosen due to its Plant Growth Promoting Rhizobacteria (PGPR) characteristics, including its capacity to produce siderophore, phosphate, ammonia, etc., Selenorhizobacteria were further applied for Vigna radiata pot study with selenium-deficient soil. MPJ12 strain was identified as Bacillus mycoides by 16S rRNA sequencing method. The strain was submitted to NCBI under accession no. MG694542. Pot study indicates there was an increase in the vegetative parameters like root and shoot length. Bacillus mycoides are capable to enhance selenium content by 0.89 ppm in Vigna radiata as well as in leaves, roots, and shoots of Vigna radiata. The use of selenorhizobacterial microbes that endorse Vigna radiata growth is becoming an effective sustainable agricultural approach to substitute synthetic fertilizers, pesticides, and supplements in selenium-deficient regions.
Keywords: Biofortification, Selenium, Selenorhizobacteria, PGPR, Vigna radiata
References
Acuna, J. J., Jorquera, M. A., Barra, P. J., Crowley, D. E., Mora, M. L. (2013). Selenobacteria selected from the rhizosphere as a potential tool for Se biofortification of wheat crops. Biology and Fertility of Soils. 49(2): 175-185.
Amato, R. D., Regni, L., Falcinella, B., Mattioli, S., Benincasa, P., Bosco, A. D., Pacheco, P., Proietti, P., Troni, E., Santi, C., Businelli, D. (2020). Current Knowledge on Selenium Biofortification to Improve the Nutraceutical Profile of Food: A Comprehensive Review. Journal of Agricultural and Food Chemistry. 68: 4075-4097.
Duran, P., Acuna, J. J., Gianfreda, L., Azcon, R., Funes-Collado, V., Mora, M. L. (2015). Endophytic selenobacteria as new inocula for selenium biofortification. Applied Soil Ecology. 96:319-326.
Duran, P., Acuna, J. J., Jorquera, M. A., Azcon, R., Borie, F., Cornejo, P., Mora, M. L. (2013). Enhanced selenium content in wheat grain by co-inoculation of selenobacteria and arbuscular mycorrhizal fungi: A preliminary study as a potential Se biofortification strategy. Journal of Cereal Science. 57(3): 275-280.
Haider, M. U., Hussain, M., Farooq, M., Ul-Allah, S., Ansari, M. J., Alwahibi, M. S., Farooq, S. (2021). Zinc biofortification potential of diverse mungbean [Vigna radiata (L.) Wilczek] genotypes under field conditions. PLoS One. 16(6): 1-14.
Hossain, A., Skalicky, M., Brestic, M., Maitra, S., Sarkar,S., Ahmad, Z., Vemuri, H., Garai, S., Mondal, M., Bhatt, R., Kumar, P., Banerjee, P., Saha, S., Islam, T., Laing, A. M. (2021). Selenium biofortification: roles, mechanisms, responses and prospects. Molecules. 26(881):1-29.
Jha, C. K., Saraf, M. (2012). Evaluation of multispecies plant growth promoting consortia for the growth promotion of Jatropha curcas L. Journal of Plant Growth Regulation. 31(4): 588-598.
Patel, P., Bhatt, S. A., Patel, H., Saraf, M. (2020a). Iron chelating bacteria: a carrier for biofortification and plant growth promotion. Journal of Biological Studies. 3(3): 111-120.
Patel, P., Trivedi, G., Bhatt, S., Patel, H., Saraf, M. (2020b). Development of sustainable biofortification strategy in Phaseolus vulgaris through Selenorhizobacteria under selenium deficient region. Bioscience Biotechnology Research Communications. 13(1): 1-9.
Patel, P., Trivedi, G., Saraf, M. (2018c). Iron biofortification in mungbean using siderophore producing plant growth promoting bacteria. Environmental Sustainability. 1(4): 357-365.
Patel, P., Trivedi, G., Shah, R., Saraf, M. (2018a). Selenorhizobacteria: As biofortification tool in sustainable agriculture. Biocatalysis and Agricultural Biotechnology. 14: 198-203.
Patel, P., Trivedi, G., Shah, R., Saraf, M. (2018b). Isolation of iron chelating bacteria and characterization of catecholate type of siderophore by HPLC analysis. Vidya – Research Journal of Gujarat University. 70-74.
Patel, P., Trivedi, G., Shah, R., Saraf, M. (2019). Rhizospheric microflora: A natural alleviator of drought stress in agricultural crops. In: Plant Growth Promoting Rhizobacteria for Sustainable Stress Management, Micro. Sustain., Vol 12, (Eds.) R. Z. Sayyed, Naveen Kumar Arora, M. S. Reddy, Springer, Rhizobacteria in Abiotic Stress Management. 1, 03-115.
Sarwar, N., Akhtar, M., Kamran, M. A., Imran, M., Riaz, M. A., Kamran, K., Hussain, S. (2020). Selenium biofortification in food crops: Key mechanisms and future perspectives. Journal of Food Composition and Analysis. 93(103615): 1-15.
Schiavon, M., Nardi, S., Vecchia, F. D., Ertani, A. (2020). Selenium biofortification in the 21st century: status and challenges for healthy human nutrition. Plant and Soil. 453:245-270.
Tamura, K., Dudley, J., Nei, M., Kumar, S. (2007). MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution. 24(8): 1596-1599.
Trivedi, G., Patel, P., Saraf, M. (2019). Synergistic effect of endophytic selenobacteria on biofortification and growth of Glycine max under drought stress. South African Journal of Botany. 134:27-35.
Yasin, M., El-Mehdawi, A. F., Anwar, A., Pilon-Smits, E. A., Faisal, M. (2015). Microbial enhanced selenium and iron biofortification of wheat (Triticum aestivum L.) – applications in phytoremediation and biofortification. International Journal of Phytoremediation. 17(4): 341-347.
Zhou, X., Yang, J., Kronzucker, H. J., Shi, W. (2020). Selenium biofortification and interaction with other elements in plants: A review. Frontiers in Plant Science. 11(586421): 1-18.
How to cite this article
Patel, P. J., Trivedi, G., Bhatt, S. and Saraf, M. (2023). Selenorhizobacteria mediated selenium biofortification in Vigna radiata under selenium deficient region – a sustainable food approach. Science Archives, Vol. 4(1),12-17. https://doi.org/10.47587/SA.2023.4103
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