Mohd. Zakir
, Narendra Kumar and Akash
1-Himalayan University, Itanagar, Arunachal Pradesh, India
2-Alpine group of Colleges, Uttarakhand, India
3-H.R. Degree College, Uttarakhand, India
dmzakirkhawaja13@gmail.com
Article History: Nov 23, 2020 / Revised: Dec 23, 2020/ Accepted: Dec 26, 2020
Abstract
In the present study, antimicrobial and phytochemical activities of the two important medicinal plants Viz. Rumex dentatus, Saussurea lappa was carried out with the help of Agar well diffusion method. The pharmacological activity of Rumex dentatus, Saussurea lappa against Escherichia coli, Bacillus amyloliquefaciens, Pseudomonas aeruginosa, Staphylococcus aureus was tested. It was recorded in our study that aqueous extract has shown the significant antibacterial activity in all the plant extracts against all the tested bacterial strains. Aqueous extract of Rumex dentatus was found to be very effective against Pseudomonas aeruginosa (35 ± 0.44 mm) whereas aqueous extract of Saussurea lappa has been recorded with highly effective against Bacillus amyloliquefaciens (31± 0.44 mm). Further, ethanol, methanol, and aqueous extract of Saussurea lappa have shown the promising antibacterial activity against Bacillus amyloliquefaciens, Pseudomonas aeruginosa, Staphylococcus aureus except in case of methanol against Escherichia coli. Further, methanol extract of Rumex dentatus has shown great antimicrobial activity that other. Methanolic extract of Rumex dentatus extracts inhibited none of the tested bacterial strains in our study. The phytochemical investigation of the various extract of Saussurea lappa showed a positive result for Saponin, Tanin, and Phenol in methanol and ethanol extract. Terpenoid, Carbohydrate, and phenol were tested positive in all the solvents for Rumex dentatus and Saussurea lappa whereas steroid, Phlobatannin, Starch, Flavonoid, Napthoquione, and Insulin were absent in all the extract of Rumex dentatus and Saussurea lappa. We can conclude from the study that Rumex dentatus and Saussurea lappa can be used as a potential source of treating different diseases due to their great antimicrobial and biological activity along with various phytoconstituents.
Keywords Saussurea lappa, Rumex dentatus, Medicinal Plants, Antimicrobial Activity
How to cite this article
Zakir, M., Kumar, N., and Akash, (2020) Antimicrobial potential and phytochemical screening of different extracts of Rumex dentatus and Saussurea lappa from Kashmir Himalaya, India. Science Archives, 1 (3), 197-201 http://dx.doi.org/10.47587/SA.2020.1320
Crossref DOI
http://dx.doi.org/10.47587/SA.2020.1320
Copyright
This is an open-access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium provided the original work is properly cited.
References
Aboaba, O. O., and Efuwape, B. M. (2001). Antibacterial properties of some Nigerian species. Biol Res Commun;13,183e8.
Ahmad, S. S., and Javed, S. (2007) Exploring the Economic Value of Underutilized Plant Species in Ayubia National Park. Pakistan Journal of Botany. 39(5),1435-1442.
Akash, Navneet, Bhandari, B. S. (2020). Ethnomedicinal plant use and practice in praditional medicine. IGI Global, USA. 1-300. doi: https://doi.org/ 10.4018/978-1-7998-1320-0.
Bauer, A. W., Kirby, W. M. M., Sherris, J. C., and Turck, M. (1966). Antibiotic susceptibility testing by standard single disc diffusion method. Am J Clin Pathol, 36;493e6.
Chandur, U., Shashidhar, S., and Chandrasekar, S. B. (2011). Bhanumathy M, Midhun T. Phytochemical evaluation and anti-arthritic activity of root of Saussurea lappa. Pharmacologia. 2, 265-9.
Cowan, M. M. (1999). Plant products as antimicrobial agents. Clin Microbiol Rev. 12, 564e82.
Drehe, D., and Junod, A. F. (1996). Role of oxygen free radicals in cancer development. Eur J Cancer 32, 30e8.
Elzaawely, A. A., Xuan, T. D., Tawata, S. (2005). Antioxidant and antibacterial activities of Rumex japonicus H OUTT. Aerial Parts. Biological and Pharmaceutical Bulletin. 28(12), 2225-30.
Girach, R. D., Khan, H., and Ahmad, M. (2003). Botanical identification of Thuhar, seldom used as Unani medicine. Hamdard Med. XLVI(1), 27e33.
Halliwell, B., and Gutteridge, J. M. C. (1985). Free radicals in biology and medicine, vol. 23. Oxford UK; Oxford University Press. 130.
Hayek, S. A., and Ibrahim, S. A. (2012). Antimicrobial activity of Xoconostle pears (Opuntiamatudae) against E s c h e r i c h i a c o l I O157:H7 in laboratory medium. Int J Microbiol; doi: 10.1155/2012/368472.
Jang, D. S., Kim, J. M., Kim, J. H., and Kim, J.S. (2005). 24-nor-Ursane type triterpenoids from the stems of Rumex japonicus. Chemical and pharmaceutical bulletin;53(12), 1594-6.
Karou, D., Savadogo, A., Canini, A., Yameogo, S., Montesano, C., Simpore, J. (2006). Antibacterial activity of alkaloids from Sidaacuta. Afr J Biotechnol 5(2), 195e200.
Kaul, M. K. (1997) Medicinal plants of Kashmir and Ladakh, temperate and cold-arid Himalaya. New Delhi; Indus Publishing Co. p. 173.
Khanahmadi, M., Rezazadeh, S. H. and Taran M. (2010). In vitro antimicrobial and antioxidant properties of Smyrnium cordifolium Boiss. (Umbelliferae) extract. Asian J Plant Sci; 9, 99e103.
Knight, J. A. (1997). Reactive oxygen species and the neurodegenerative disorders. Ann Clin La Sci; 27, 11e25.
Kritikar, K. R., and Basu, B. D. (1987). Indian Medicinal Plants. In: Compositae, International Book Distributors, Dehradun; 2, 1420-1423.
Küpeli, E., Aslan, M., Gürbüz, I., and Yesilada, E. Evaluation of in vivo biological activity profile of isoorientin. Z. Naturforsch, 59 (30), 787-790.
Madhuri, K., Elango, K., Ponnusankar, S., Madhuri, K., Elango, K., and Ponnusankar, S. (2012). Saussurea lappa (Kuth root): review of its traditional uses, phytochemistry and pharmacology, Orient Pharm Exp Med, 12, 1. https://doi.org/10.1007/s13596-011-0043-1.
NCCLS. (1990). National Committee for Clinical Laboratory Standards Approved standard M7-A2. Standard methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically, Villanova.
Negi, J. S., Bisht, V. K., Bhatt, V. P., Sati, M. K., Mohanty, J. P., Sundriyal, R. C. (2013). Antidiarrheal activity of methanol extract and major essential oil contents of Saussurea lappa Clarke. Afr J of phar and pharma 28, 7(8), 474-7.
Qureshi, N. N., Kuchekar, B. S., Logade, N. A., and Haleem, M. A. (2009) Antioxidant and hepatoprotective activity of Cordia macleodii leaves. J Saudi Pharm; 17(4), 317e32.
Rao, K. S., Babu, G. V., Ramnareddy, Y. V. Acylated flavone glycosides from the roots of Saussurea lappa and their antifungal activity. Molecules. 2007 Mar;12(3), 328-44.
Sarin, Y. K. (1996). Illustrated manual of herbal drug used in ayurveda. New Delhi: CSIR and ICMR. Niscom.
Shah, N. (1982). Herbal folk medicines in Northern India. Journal of Ethnopharmacology; 6 (3), 293-301.
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