Abeer Mahmood.AL-Sabaawy¹✉
, Enas Abdul-muniem. AL-Layla¹, Omar Ibrahim. AL-Sabaawy²
¹Department of Biology, College of Science, University of Mosul, Mosul, Iraq
²AL-Salam Teaching Hospital, Iraq
Received: Sept 2, 2022/ Revised: Oct 1, 2022/ Accepted: Oct 2, 2022
(✉) Corresponding Author: abeer2022mf@gmail.com
Abstract
Chalazion is a common infection of the meibomian gland in all ages’ eyelids. The current study sought to isolate and identify bacteria linked with chalazion in patients hospitalized to Mosul hospitals between 10/1/2021 and 1/3/2022. Samples were obtained using swabs soaked in the transport media (amies), and bacteria were identified using traditional methods with API20E. Vitek-2 and molecular approaches based on the 16 S rRNA gene verified the species-level diagnosis. Using BLAST software, the nitrogen base sequences were determined and compared to those of the NCBI. The results showed that Staphylococcus aureus was isolated by 52.2% and 62.5% among women, notably in the age (21-40) years, and by 27.8% for females and 19.2% for the same age group in males, and upper eyelid infection predominated. The infection rate in people with oily skin was 50.9% in females and 34.6% in men, with the presence of the agr A, agrC, and agrD genes in isolates that exhibited a positive result in the Congo red medium and microtiter plate.
Keywords: Chalazion, Miebomain gland, Staphylococci, Agr genes
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How to cite this article
AL-Sabaawy, A. M., AL-Layla, E. A., AL-Sabaawy, O. I. (2022). Detection of bacterial infection of the eye chalazion in Mosul City. Science Archives, Vol. 3(4), 240-246. https://doi.org/10.47587/SA.2022.3401
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